Recombinant Human Anti-H3N2 HA Antibody (F045-092)
CAT#: PABC-233
Recombinant Human Antibody (F045-092) is capable of binding to H3N2 HA, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-H3N2 HA mAb and CH1-3 region of human IgG and a light chain (LC) encoding VL from anti-H3N2 HA proteins mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition. The antibody, F045-092, that possesses broadly neutralizing activity against the entire H3 subtype and accommodates.
Tested Data
SDS-PAGE
Figure 1 Recombinant Human Anti-H3N2 HA Antibody (F045-092) (PABC-233) in SDS-PAGE
SDS-PAGE analysis of PABC-233 In non-reduced (lane 1) and β-mercaptoethanol-reduced (lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively. And non-reduced protein migrates as 130-180 kDa.
ELISA
Figure 2 Recombinant Human Anti-H3N2 HA Antibody (F045-092) (PABC-233) in ELISA
ELISA analysis of PABC-233 was performed by coating with Influenza A H3N2 (A/Perth/16/2009) Hemagglutinin / HA Protein (His Tag).
The secondary antibody: HRP-Anti-Human IgG (H+L)
ELISA
Figure 3 Recombinant Human Anti-H3N2 HA Antibody (F045-092) (PABC-233) in ELISA
ELISA analysis of PABC-233 was performed by coating with Influenza A H3N2 (A/Victoria/210/2009) Hemagglutinin / HA Protein (His Tag).
The secondary antibody: HRP-Anti-Human IgG (H+L)
ELISA
Figure 4 Recombinant Human Anti-H3N2 HA Antibody (F045-092) (PABC-233) in ELISA
ELISA analysis of PABC-233 was performed by coating with Influenza A H2N2 (A/Japan/305/1957) Hemagglutinin / HA Protein (His Tag).
The secondary antibody: HRP-Anti-Human IgG (H+L)
WB
Figure 5 Recombinant Human Anti-H3N2 HA Antibody (F045-092) (PABC-233) in WB
Western blot analysis of PABC-233 was performed by loading Influenza A H3N2 (A/Perth/16/2009) Hemagglutinin / HA Protein (His Tag), Influenza A H3N2 (A/Victoria/210/2009) Hemagglutinin / HA Protein (His Tag), Influenza A H2N2 (A/Japan/305/1957) Hemagglutinin / HA Protein (His Tag).
PABC-233 incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Anti-Human IgG (H+L)
Left: Reducing Influenza A H3N2 (A/Perth/16/2009) Hemagglutinin / HA Protein (His Tag) (0.8 μg)
Middle: Influenza A H3N2 (A/Victoria/210/2009) Hemagglutinin / HA Protein (His Tag) (0.8 μg)
Right: Influenza A H2N2 (A/Japan/305/1957) Hemagglutinin / HA Protein (His Tag) (0.8 μg)
DB
Figure 6 Recombinant Human Anti-H3N2 HA Antibody (F045-092) (PABC-233) in DB
Dot Blot analysis of PABC-233 was performed by coating with Influenza A H3N2 (A/Perth/16/2009) Hemagglutinin / HA Protein (His Tag), Influenza A H3N2 (A/Victoria/210/2009) Hemagglutinin / HA Protein (His Tag), Influenza A H2N2 (A/Japan/305/1957) Hemagglutinin / HA Protein (His Tag).
PABC-233 incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Anti-Human IgG (H+L)
Top: Reducing Influenza A H3N2 (A/Perth/16/2009) Hemagglutinin / HA Protein (His Tag)
Middle: Influenza A H3N2 (A/Victoria/210/2009) Hemagglutinin / HA Protein (His Tag)
Bottom: Influenza A H2N2 (A/Japan/305/1957) Hemagglutinin / HA Protein (His Tag)
❮
❯
Specifications
- Host Species
- Human
- Derivation
- Human
- Type
- IgG
- Specificity
- Tested positive against native H3N2 HA
- Species Reactivity
- H3N2
- Clone
- F045-092
- Applications
- Can be useful in applications such as: Neutralization
Product Property
- Purity
- >95% by SDS-PAGE and HPLC analysis
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Target
- Alternative Names
- Hemagglutinin; HA; H3N2; Influenza A virus
- UniProt ID
- R9U684
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(Creative Biolabs Cat# PABC-233, RRID: AB_3111579)
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
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